International supplier for the biopharmaceutical industry, Sartorius Stedim Biotech (SSB) has expanded its range of single-use membrane chromatography solutions with Sartobind Cassettes. This convenient, pod-like modular system has been developed for commercial applications in both capture and polishing.

New Sartobind cassettes offer the same flow path, bed heights (4 and 8 mm) and void volume ratios as Sartobind capsules. Multiple cassettes, each with 0.8 L or 1.6 L membrane volume, can be set up in three different stainless steel holders resulting in maximum membrane volumes of 20, 50 or 100 L, respectively. Pressure-flow performance and the shape of breakthrough curves are identical to the smaller capsule sizes, independent of the number of cassettes used. Set up can be accomplished within minutes, even at manufacturing scale, whether for capture applications or for flow-through removal of process contaminants.

Capturing of large proteins like viruses and virus like particles (VLPs), protein conjugates and blood factors that plays a key role in modern bioprocessing. The unique Sartobind cassette system now enables large-scale bind-and-elute membrane chromatography for such targets

Dr. Stefan Fischer-Frühholz, membrane chromatography expert at Sartorius Stedim Biotech said, “For the first time, the high binding capacity of membrane adsorbers can be used at almost any scale in commercial vaccine manufacture. Now, a 200 L Q anion exchange column for adenovirus capture can be easily replaced by a 20 L Q adsorber cassette system”