Updated on 14 February 2013
Sigma Life Science provides the TuD RNAs in both synthetic and lentiviral formats to support transient miRNA knockdown as well as long-term miRNA suppression without repeated transfections. The miRNA binding sites are designed using human and mouse sequence data from the most recent version of miRBase.
Many other tools for miRNA screening, identification, and validation experiments are available from Sigma Life Science. These include synthetic human miRNA mimics, a miRNA isolation kit, a method to identify the specific gene(s) that a miRNA targets (licensed exclusively from Dr Joop Gaken at King's College London, and a library of human 3′UTRs for validating many miRNA gene targets.